recombinant human cd40l (R&D Systems)

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R&D Systems recombinant human cd40l
Recombinant Human Cd40l, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+cd40+ligand/Recombinant+Human+CD40+Ligand%2FTNFSF5+(Histidine-tagged)/pmc12901160-269-77-82
Average 94 stars, based on 16 article reviews

recombinant human cd40l - by Bioz Stars, 2026-09

94/100 stars

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Activation Assay:

Article Title: Interplay between the Epigenetic Enzyme Lysine (K)-Specific Demethylase 2B and Epstein-Barr Virus Infection
Article Snippet: Anti-miRNA treatment was done by the addition of Hsa-miR-146a-5p miRCURY LNA miRNA power inhibitor (250 nM; Qiagen) directly to the cell culture medium 24 h before infection with EBV. .. To induce their activation, primary B cells were seeded at a density of 0.5 × 10 6 cells in 6-well dishes and treated with 100 ng/ml recombinant human CD40 ligand (hCD40L; catalog number 6245-CL; R&D Systems) and 20 ng/ml of recombinant human IL-4 (R&D Systems) for 48 h. Cells were then collected and processed for further analysis. .. To block DNA methylation, cells were treated with 5-aza-2′-deoxycytidine (≥97%; catalog number A3656; Sigma-Aldrich) at a final concentration of 10 μM for 48 or 96 h. To inhibit the different pathways, cells were treated with the IκBα kinase inhibitor BAY11-7082 (Calbiochem) at a final concentration of 10 μM or with the JNK inhibitor II SP600125 (catalog number 420119; VWR International), used at a final concentration of 10 μM.

Article Title: Abnormalities of bone marrow B cells and plasma cells in primary immune thrombocytopenia
Article Snippet: In vitro Breg suppression assays Function of Bregs was evaluated as previously reported by Vlkova and Iwata et al.1, 2 B cells were selected from BMMNCs by human CD19 microbeads (Miltenyi Biotec). .. Purified (> 95%) CD19+B cells were stimulated with 200 nM CpG-ODN and 50 μg/ml recombinant human CD40 ligand (R&D Systems) for 24 hr to induce Breg activation. ..

Recombinant:

Article Title: Immunostimulatory Activity of the Cytokine-Based Biologic, IRX-2, on Human Papillomavirus-Exposed Langerhans Cells
Article Snippet: .. Recombinant human CD40 ligand (R&D Systems) was used as a positive control to confirm LC responsiveness. ..

Article Title: A proliferation-inducing ligand sustains the proliferation of human naïve (CD27⁻) B cells and mediates their differentiation into long-lived plasma cells in vitro via transmembrane activator and calcium modulator and cyclophilin ligand interactor and B-cell mature antigen.
Article Snippet: For cell culture, recombinant human IL-21 was purchased from Miltenyi Biotech. .. Recombinant human APRIL, recombinant human BAFF, histidine-tagged soluble recombinant human CD40 ligand, and an antipolyhistidine mAb were from R&D Systems (Minneapolis, MN, USA). .. Phosphorothioate CpG-ODN 2006 was purchased from Invivogen (San Diego, CA, USA) and Ara-C (cytosine arabinoside) from Sigma–Aldrich.

Article Title: Amplification of Toll-like receptor-mediated signaling through spleen tyrosine kinase in human B-cell activation.
Article Snippet: Abbreviations used AICDA: Activation-induced cytidine deaminase BCR: B-cell receptor FITC: Fluorescein isothiocyanate NF-kB: Nuclear factor kB PI: Propidium iodide SLE: Systemic lupus erythematosus Syk: Spleen tyrosine kinase TLR: Toll-like receptor TRAF: TNF receptor–associated factor XBP-1: X-box binding protein 1 Background: B cells are activated by combined signals through the B-cell receptor (BCR) and CD40.. However, the underlying mechanisms by which BCR signals synergize with Toll-like receptor (TLR) signaling in human B cells remain unclear.. Objective: We sought to elucidate a role of spleen tyrosine kinase (Syk), a key molecule of BCR signaling, in TLR-mediated activation of human B cells.

Article Title: Lysosome-Disrupting Agents in Combination with Venetoclax Increase Apoptotic Response in Primary Chronic Lymphocytic Leukemia (CLL) Cells Mediated by Lysosomal Cathepsin D Release and Inhibition of Autophagy
Article Snippet: .. Cathepsin inhibitor E-64 (Thermofisher, Mississauga, ON, Canada AAJ62933LB0) was dissolved in DMSO, ɑ-tocopherol (Sigma, St. Louis, MO, USA 258024-100G) was dissolved in ethanol, recombinant human IL-4 protein (R&D systems, Minneapolis, MN, USA BT-004), and recombinant human CD40 ligand (R&D systems, Minneapolis, MN, USA 6420-CLB) were dissolved in PBS, as per the manufacturer’s instructions. .. Stains were purchased from the following suppliers: trypan blue solution (Sigma, St. Louis, MO, USA T8154), LysoTracker Red DND-99 (Thermofisher, Mississauga, ON, Canada L7528), Dihydroethidium (DHE), MitoSox Red (Thermofisher, Mississauga, ON, Canada M36008), DAPI (Sigma, St. Louis, MO, USA D9542), Annexin V FITC (BD Bioscience, Milptitas, CA, USA 556419), and 7-AAD (BD Bioscience, Milpitas, CA, USA559925).

Positive Control:

Purification:

other:

Article Title: Diverse mutational landscapes in human lymphocytes
Article Snippet: Fluorescence activated cell sorting • Fetal bovine serum (Thermo Fisher Scientific, cat. no. 10270-106) • Phosphate buffered saline (Sigma-Aldrich, cat. no. D8537-500ML) • APC anti-human CD3, clone Hit3a (Tonbo Biosciences, cat. no. 20-0039-T100) • Brilliant violet 785 anti-human CD4, clone OKT4 (BioLegend, cat. no. 317442) • BV650 mouse anti-human CD8, clone RPA-T8 (BD Biosciences, cat. no. 563821) • Alexa Fluor 700 anti-human CD19, clone HIB19 (BioLegend, cat. no. 302226) • PE/Dazzle 594 anti-human CD20, clone 2H7 (BioLegend, cat. no. 302348) • BV421 mouse anti-human CD27, clone M-T271 (BD Biosciences, cat. no. 562513) • FITC anti-human CD38, clone HIT2 (BioLegend, cat. no. 303504) • PerCP/Cyanine5.5 anti-human CD45RA, clone HI100 (BioLegend, cat. no. 304122) • BV711 anti-human CD197 (CCR7), clone G043H7 (BioLegend, cat. no. 353228) • PE/Cyanine7 anti-human IgD, clone IA6-2 (BioLegend, cat. no. 348210) • Zombie Aqua Fixable Viability Kit (BioLegend, cat. no. 423102) • BD Comp Beads, anti-mouse Ig, κ (BD Biosciences, cat. no. 51-90-9001229) • BD Comp Beads, negative control (BD Biosciences, cat. no. 51-90-9001291) Cell culture • Advanced RPMI 1640 Medium (Sigma-Aldrich, cat. no. R0883-500ML) • ImmunoCult-XF T Cell Expansion Medium (Stem Cell Technologies, cat. no. 10981) • Fetal bovine serum (Thermo Fisher Scientific, cat. no. 10270-106) • Penicillin/streptomycin (Sigma-Aldrich, cat. no. P4333-100ML) • L-glutamine (Sigma-Aldrich, cat. no. G7513-100ML) • Anti-IgM (Stratech Scientific Limited, cat. no. 109-006-129-JIR-1mg) • Recombinant human IL-2 (PeproTech, cat. no. 200-02-500) • Recombinant human IL-4 (PeproTech, cat. no. 200-04-50) • Recombinant human IL-15 (PeproTech, cat. no. 200-15-50) • Recombinant human IL-21 (PeproTech, cat. no. 200-21-100) • CD40L-HA, recombinant human CD40 ligand (R&D Systems, cat. no. 6420-CL-025) • HA tag antibody (R&D Systems, cat. no. MAB060) • ImmunoCult Human CD3/CD28 T cell activator (Stem Cell Technologies, cat. no. 10971) Sample digest • Arcturus PicoPure DNA extraction kit (Thermo Fisher Scientific, cat. no. KIT0103)

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